| abstract |
A novel CRISPR-associated transposase derived from pseudoalteromonas translucida KMM 520, wherein 16 PAMs can be identified and cargo genes can be inserted into 8 sites at a time with an efficiency of 100%, higher than a CRISPR-associated transposase derived from vibrio cholerae Tn6677; the transposition of the 15.4kb cargo gene to the corresponding target site has an efficiency of 100%, and the present transposase and the CRISPR-associated transposase derived from vibrio cholerae Tn6677 can be used in the same E. coli and function without interfering with one another, having broad prospects for application. |