| abstract |
A preparation method for a polypeptide, the preparation method comprising the following steps: constructing an engineering strain of a Sumo tag fusion expression polypeptide gene, and inducing the engineering strain for the soluble expression of a polypeptide; purifying a crude protein containing a polypeptide precursor from the engineering strain; digesting the crude protein containing the polypeptide precursor by using Ulp1 protease to remove the Sumo tag; purifying a cleavage product of the Ulp1 protease by means of acetonitrile combined with heating precipitation or by using a method for hexafluoroisopropanol precipitation, so as to obtain the polypeptide. Efficient technology based on the soluble recombinant expression of a medicinal polypeptide or a precursor thereof has been established, a simple purification process based on one-step precipitation purification has been established, and further in combination with HPLC purification, a polypeptide purity of more than 97% can be achieved. |