| abstract |
Disclosed herein include methods, compositions, kits and systems for multiplexed unbiased nucleic acid amplification. In some embodiments, the methods, composition, kits and systems enable the use of a single primer or a single primer pair for amplifying multiple nucleic acid targets. There are provided, in some embodiments, a plurality of protein complexes. Each protein complex can comprise a transposome and a programmable DNA binding unit capable of specifically binding to a user-selected binding site on a target double-stranded DNA (dsDNA). The binding site for each of the plurality of protein complexes can be different from each other. The transposome can comprise a transposase and two copies of an adaptor. |