Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6f4546468d57daf4607bbd7e2904732 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d5cd84f92e5e2ff95dfd19e4e329db3b |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2022-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8182404de7d6125771fc1e7e566b493d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc2e7ea2ae6cbdf6dfb98f2d8cae6d89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40f75a1f63671709687862e49544bc4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44f4d1ea68d2122250dcdb6f8f7d3c99 |
publicationDate |
2022-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2022240960-A1 |
titleOfInvention |
Mrna large scale synthesis and purification |
abstract |
Described herein is method for purifying messenger RNA (mRNA) encoding a DNA endonuclease from a sample, the method comprising: (a) loading the sample comprising the mRNA onto a monolithic matrix comprising a poly(dT) or poly(U) nucleic acid molecule linked/coupled to the monolithic matrix under conditions allowing the mRNA to hybridize with the poly(dT) or poly(U) nucleic acid molecule; (b) eluting the mRNA from the monolith matrix after one or more contaminants have been separated from the bound mRNA; and (c) separating the mRNA from dsRNA by adsorption chromatography, thereby resulting in a purified mRNA solution. |
priorityDate |
2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |