http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022239632-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69b4de7d64ebafc368b849bca94eab18 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38fc74202f24d24413ab2a6c83935d83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d2e83bd29877fb7717e44c0fc629d9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f81565b2023bc4ff446e1a661cceec39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e08f45b4c79ba16e6592264c4bff6d8 |
| publicationDate | 2022-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022239632-A1 |
| titleOfInvention | Method for producing synthesized dna molecule |
| abstract | This method for producing a synthesized DNA molecule constituted of a desired sequence includes: a step for preparing a plurality of template oligonucleotides each having a portion of the desired sequence; a step for preparing a plurality of splint oligonucleotides each having, when the plurality of template oligonucleotides are arranged in a desired sequence, a sequence complimentary to the sequence of the end portions of the adjacent oligonucleotides; a step for annealing the plurality of splint oligonucleotides to the plurality of template oligonucleotides; a step, which is carried out after the step for annealing, for ligating the plurality of template oligonucleotides to one another, and synthesizing a circular single-strand DNA that includes the plurality of splint oligonucleotides as complementary strands; and, with the circular single-strand DNA as the template, a step for synthesizing a double-strand DNA using φ29 DNA polymerase. |
| priorityDate | 2021-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.