http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022237035-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_970d0335eb6be0526b49b2c2d8713c5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80360dda9a50a60a5df3841df3bfd5d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be10bfd98ef0e3ae624944e32cfdd115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_660414ba405ef79e480a1de94c6c6698 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad06d53cdffd64405cfe895f7f546a13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73b3147dc8b141560cb615a9ba44e081 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_987cf82c0782d0e374d93b4bb8f652ab |
| publicationDate | 2022-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022237035-A1 |
| titleOfInvention | Ultra-high-throughput single-cell sequencing method |
| abstract | Disclosed is an ultra-high-throughput single-cell sequencing method. In the ultra-high-throughput single-cell sequencing method of the present invention, a reverse transcription sequence is first used to perform one instance of intracellular reverse transcription within cells, or transposition of a chromatin transposase-accessible genome sequence within nuclei is first performed by using a specific molecular tag transposase-embedded complex; then, the cells or cell nuclei pass through microplate technology or microfluidic technology, such that one molecularly labeled microbead and one or more cells are in a separate space, and the cells/cell nuclei are lysed under the action of a lysate; with the help a bridging primer; a sequence is linked to a molecular marker sequence on molecular marker microbeads, and a large number of sequences are obtained by means of PCR amplification for construction so as to obtain a cDNA sequencing library; and then high-throughput sequencing is carried out, wherein specific transcriptome/genome openness information of millions of single cells can be obtained in one instance of sequencing. The throughput of single cell sequencing is greatly improved. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116564415-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116564415-B |
| priorityDate | 2021-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.