| abstract |
The present invention relates to the technical field of biochemical detection, and in particular relates to a preparation method for alkaline phosphatase-labelled thyroxine. In the preparation method provided in the invention, after being activated, alkaline phosphatase is directly linked to thyroxine without the need to activate T4 and without the need to couple macromolecules such as BSA. The prepared ALP-T4 complex has good activity and does not contain by-products. The preparation method has simple processes and is low cost. It has been verified that the ALP-T4 prepared using the present method detects free thyroxine or total thyroxine with good sensitivity, accuracy, precision, and specificity. |