| abstract |
The invention relates to a method for inserting a plurality of copies of a gene of interest into the genome of a fungus, said method comprising a step of transforming said fungus using a vector comprising an expression cassette, wherein said expression cassette comprises: (1) a TEF1 promoter identical to the endogenous TEF1 promoter of the species of fungus, (2) a gene of interest, and (3) a terminator, with the exception of the endogenous TEF1 terminator of the species of fungus. The invention also relates to the fungi obtained using such a method, and to the various uses of the strains thus modified. |