| abstract |
The present invention provides a construction method for an RNA sequencing library, a sequencing method, and a kit. The construction method comprises: acquiring single-stranded cDNA, i.e., a reverse transcription product, of mRNA, the 3'-end of the single-stranded cDNA containing a cDNA tag sequence; cyclizing the single-stranded cDNA to obtain a single-stranded cyclized cDNA; amplifying the single-stranded cyclized cDNA by using a primer combination formed by a random primer or a gene-specific primer and a cDNA tag primer to obtain amplified fragments, the cDNA tag primer being at least a part of the cDNA tag sequence; and performing fragmentation-based library construction on the amplified fragments to obtain an RNA sequencing library. |