| abstract |
Methods of determining the presence or absence or local concentration of an analyte in a sample or an individual or a portion thereof using ratiometric sensing and optical super-resolution microscopy (OSRM). The methods use aluminosilicate nanoparticles that can be used in OSRM. The analytes can be biologically relevant analytes, such as, for example, biologically relevant hydrogen ions, oxygen, reactive oxygen species, anions, nitric oxide, metal ions, anions, etc. The methods utilize averaging to address aluminosilicate nanoparticle homogeneity. The methods can be used in methods of treatment. |