http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022211674-A1

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
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filingDate 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_270c3c84b9b2f773676e4081728241f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5894b77dd88931705c2fc0388bcce0ca
publicationDate 2022-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2022211674-A1
titleOfInvention Method of cultivating aerobic methane-assimilating microorganisms
abstract The invention relates to biotechnology. The invention relates to biotechnology. Proposed is a method of cultivating aerobic methane-assimilating Methylococcus capsulatus microorganisms in a continuous flow mode with a constant rate of culture fluid removal and with a mineral medium flow rate of from 0.25 h-1 to 0.35 h-1, at 41-44°C, a pH of 5.4-5.8 and an optimal flow rate of a gas-air mixture of methane:air in a ratio of 1:3, wherein, with respect to the composition of the nutrient medium, nickel is additionally added and up to 10% of water is replaced by culture medium centrate; the concentration of the components of the mineral feed is determined according to the given flow rate D of the medium and the planned yield Y of the cultivation process, using a specific formula. The invention provides for an increase in the yield of a process for synthesizing a biomass in bioreactors with different mass transfer characteristics, while scaling up the process of prolonged cultivation.
priorityDate 2021-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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