http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022205578-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf6ea5ed9ddb34cd21f92f6a79595263 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00313 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00686 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e266d16b848ba11ddff17d76483ca795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8cd73d9a186e580dd25a04b7ded7ccc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb13fdfde9234b360cae299c59b8566b |
| publicationDate | 2022-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022205578-A1 |
| titleOfInvention | High-throughput automated gene synthesis apparatus based on cluster array |
| abstract | A high-throughput automated gene synthesis apparatus based on a cluster array. The high-throughput gene synthesis apparatus comprises a substrate and a microwell plate, wherein the substrate is provided with several clusters of microwells (1); a sidewall surface of the microwell (1) is chemically modified and is then used as a solid-phase carrier for nucleic acid synthesis, or the microwell (1) is filled with solid-phase carriers; and the several clusters of microwells (1) are arranged in a cluster array, each cluster of microwells (1) has the same size as that of each hole (2) on the microwell plate, and the position of each cluster of microwells (1) corresponds to the position of each hole (2). When oligonucleotides are synthesized using the apparatus, the synthesized oligonucleotides are automatically recovered into a standard SBS plate (a 96-well plate, a 384-well plate, a 1536-well plate, etc.) having a corresponding size under the apparatus, thereby forming oligonucleotide pools required for each gene. The yield of the oligonucleotides is at a pmoL level, and is sufficient for direct subsequent gene assembly by means of polymerase cycling assembly (PCA) or ligase chain reaction (LCR) without amplification. The oligonucleotides which have been subjected to error correction can thus be used for full-length gene splicing, thereby realizing high-throughput automated gene synthesis. |
| priorityDate | 2021-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.