http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022199242-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f312cc7676e939dee48fb4fdfe921ce8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_888eb5d8da64921073d3a7e0bd93f5c8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate | 2022-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee441bc98ad165fe49d3078a8402f9fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6aef5b1515ebd1a8ec0c31e8b0cfb427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81582acca761fe774c6f55ddb1e31c6f |
| publicationDate | 2022-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022199242-A1 |
| titleOfInvention | Set of barcode linkers and medium-flux multi-single-cell representative dna methylation library construction and sequencing method |
| abstract | Provided is a set of adhesive linkers comprising sample barcodes, for use in specific labeling of different samples. Each linker is formed from a short oligonucleotide and a long oligonucleotide, and unique barcode sequences are set for different linkers. The linker is directly connected to the end of a restriction genomic DNA fragment, and is used for labeling a plurality of single cells or population cells or purified DNA samples and performing amplification thereof. Also provided are a method for simultaneously detecting CpG methylation of a plurality of samples, briefly referred to as M-scRRBS, and an alternative method thereof, i.e., M-scRRAS. The method comprises: using the linkers to specifically label a plurality of samples, comprising all DNA fragments of each sample, then combining the plurality of samples to achieve a single-tube reaction of the plurality of samples, performing subsequent transformation, sequencing library construction and sequencing, and sample reading separate decoding and downstream analysis. Compared with the scWGBS and scRRBS methods, the library construction technology has the advantages of high efficiency, low cost, stable and convenient operation and the like. |
| priorityDate | 2021-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 106.