http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022185928-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_feacfe1b388ddf96ee35ca8a9e710eb4 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2022-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41ea88248b94fde7d5df6f33583dd9a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_568ebb8753437737dda96fc307d5b39c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a325359bbdd7bd3495698c266396b10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fefafa738134e3d4d8faad909e405cce |
publicationDate | 2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2022185928-A1 |
titleOfInvention | Method for evaluating cystine uptake ability of cell, kit for evaluating cystine uptake ability of cell, method for determining selenocysteine, and kit for determining selenocysteine |
abstract | Disclosed are: a method for evaluating the cystine uptake ability of a cell having a step that brings selenocystine into contact with a cell and causes selenocystine to be taken up by the cell, a step for washing away the selenocystine that has not been taken up by the cell, and a step for crushing the cell and determining the selenocystine included in the cytoplasm; and a method for determining selenocysteine having a step for bringing selenocysteine into contact with a fluorescent dye that reacts specifically with selenocysteine to change the fluorescence wavelength and/or fluorescence intensity or reacting selenocysteine with a fluorescent dye under conditions such that the fluorescent dye reacts specifically with selenocysteine to change the fluorescence wavelength and/or fluorescence intensity, and determining the selenocysteine by measuring the fluorescence intensity. Also disclosed is a kit for evaluating the cystine uptake ability of a cell that includes a reducing agent for reducing selenocystine to selenocysteine, a fluorescent dye represented by general formula (I), and a pH 5.5-6.5 buffer. |
priorityDate | 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.