| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f0637711c843740f46a8f93225544864 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-052
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2313
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-20051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2304
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P29-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0786
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P37-04 |
| filingDate |
2021-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e2c8fe26537948770ec0e363b50870a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85c26cf6a61c28d1ff58587a06d81744
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8812c7bea422aae31a8bf87246782b52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4c2b38461177b438878e72277b2f5c5 |
| publicationDate |
2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2022183593-A1 |
| titleOfInvention |
Porcine alveolar macrophage healthy cell line, and construction method and application |
| abstract |
Provided are a porcine alveolar macrophage healthy cell line, and a construction method and an application. The porcine alveolar macrophage healthy cell line has an accession number of CGMCC No: 21498. In the porcine alveolar macrophage healthy cell line, a 100% healthy porcine alveolar macrophage cell line is screened by using a special cell culture solution in combination with a natural domestication selection method; the porcine alveolar macrophage healthy cell line can be applied to cell culture of viruses, research and development and production of live vaccines and inactivated vaccines and preparation of bioactive substances secreted by M1 and M2 macrophages after induced polarization to be respectively used for cancer treatment, inflammation diminishing and the like. The construction method provided does not employ any carcinogenic substances such as lentivirus transformation or plasmid transfection, and the constructed porcine alveolar macrophage healthy cell line keeps the characteristics of primary porcine alveolar macrophages. For example, in terms of sensitivity to porcine virus infection, the increasingly difficult research of using a Marc145 cell line to separate and culture a newly emerging porcine reproductive and respiratory syndrome virus, and using a Vero cell line to separate and culture a newly emerging virus such as a newly emerging porcine epidemic diarrhea virus mutant strain becomes easier. |
| priorityDate |
2021-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |