| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7428b567e9dad4674774969d58f64fbf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f473b0907ef6d2eb66121bbe11e26ccf |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0028
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-03001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-03009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-03003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y105-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-0104
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 |
| filingDate |
2021-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c771689f73d0a7918df54ce8b04666d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b4fb9facd93d20fc48fa24d4f82a7e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_397b48feac4a43a71cb21d71ab9293f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a601b58a3361a92e4be7df9d54d126c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3e6d6649dab37824d5227b6c3663d3f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_425f7d15502c2043c587e0d3de741b54 |
| publicationDate |
2022-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2022174597-A1 |
| titleOfInvention |
Genetically engineered bacterium for producing l-sarcosine, construction method therefor and use thereof |
| abstract |
Provided are a genetically engineered bacterium for producing L-sarcosine, a construction method therefor and the use thereof. The genetically engineered bacterium takes escherichia coli as a host, and is obtained by means of integrating the following steps on the genome thereof: single copying an imine reductase gene dpkA; single copying a citrate synthase gene gltA; knocking out a glyoxylate cycle-inhibiting gene iclR; knocking out a malate synthase gene aceB; single copying an isocitrate lyase gene aceA; single copying a membrane-bound transhydrogenase gene pntAB; knocking out 2-keto acid reductase gene ycdW; single copying a phosphoenolpyruvate carboxylase gene ppc; and knocking out a pyruvate kinase gene pykF. After systemic metabolic modification is performed, the genetically engineered bacterium can be used for synthesizing L-sarcosine by means of taking glucose and methylamine as the main raw materials. After fermentation is performed in a 5 L fermentation tank for 30 h, the yield of L-sarcosine can reach 10 g/L. |
| priorityDate |
2021-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |