| abstract |
The present application relates to a preparation method for tumor-infiltrating lymphocytes (TILs) and a use thereof, in particular, to a method for culturing TILs, comprising: subjecting TILs derived from tumor tissue and not expanded in vitro to at least one stage of in vitro expansion, wherein in the at least one stage of in vitro expansion, the TILs are made to contact one or more T cell activators and one or more immune checkpoint inhibitors. A method for preventing and/or treating a tumor by using the TILs is further provided. |