| abstract |
Disclosed herein are allosteric conditional gRNA (cgRNA) mechanisms for both ON->OFF logic (conditional inactivation by an RNA trigger) and OFF->ON logic (conditional activation by an RNA trigger). Allosteric cgRNAs enable restriction of CRISPR/Cas function to a desired cell type, tissue, organ, or disease state. Allosteric cgRNAs provide a versatile platform for cell-selective and tissue-selective research tools, biotechnologies, diagnostics, and therapeutics. |