abstract |
Provided are a method for treating a cell or a nucleus to produce a population of nucleic acid fragments, and a method for generating a labeled nucleic acid molecule by means of using the population of nucleic acid fragments, constructing a library of nucleic acid molecules for transcriptome sequencing, or performing high-throughput sequencing on a single-cell transcriptome. Further provided are a library of nucleic acid molecules constructed by means of using the method, and a kit for carrying out the method. |