http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022129222-A1

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filingDate 2021-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cb95ba4cebff7b8795fa0c968698746
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publicationDate 2022-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2022129222-A1
titleOfInvention Method for optically clearing a tissue sample using an embedding medium
abstract The invention relates to a method for producing transparent tissue samples of a biological or human tissue for a light microscopy examination, having the steps a) dewatering the tissue sample using a dewatering solvent and b) clearing the dewatered tissue sample by transferring same into an embedding medium which contains an benzaldehyde-anisole ether. In comparison to the benzyl benzoate/benzyl alcohol mixtures and methyl salicylate-benzyl benzoate mixtures conventionally used until now as embedding media, the benzaldehyde-anisole ether has the advantage of being usable as a pure substance, in exactly the same way as a dibenzyl ether: Because the pure substance already has the desired refractive index, the refractive index does not need to be set by mixing the embedding medium. Furthermore, the benzaldehyde-anisole ether according to the invention penetrates the dewatered tissue faster than previously known embedding media and also makes the tissue transparent faster.
priorityDate 2020-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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