| abstract |
Provided in the present application is a chimeric antigen receptor, including a GPC3 antigen binding domain, an ICD1, ICD2 or ICD3 intracellular signal stimulation domain with amino acid sequences of SEQ ID NOs: 29, 31 and 33, respectively, and an IL-15-IL-15α fusion protein with an amino acid sequence of SEQ ID NO:7. After the chimeric antigen receptor is transferred into immune cells, especially iNKT cells, the cell proliferation rate, survival time and tumor killing effect can be effectively improved. Further provided in the present application are a corresponding expression vector, a transduction system, a pharmaceutical use, independent ICD1, ICD2 and ICD3 intracellular signal stimulation domains, and an IL-15-IL-15α fusion protein. |