| abstract |
This disclosure relates to methods for enhancing the therapeutic efficacy of isolated cells for use in cell therapies such as adoptive cell transfer therapies by insertion of an under¬ expressed miRNA that is beneficial for therapeutic efficacy of cell therapies into the actively expressed locus of a gene, either protein coding or non-coding, that hampers therapeutic efficacy of cell therapies by this disrupting expression of the latter while inducing expression of the former. |