| abstract |
Provided herein are engineered guide RNAs comprising structured loop stabilized scaffolds and engineered guide RNAs configured, upon hybridization to a target RNA, to form, at least in part, double stranded substrates with at least a portion of the target RNA and facilitate a chemical modification of a nucleotide base in the target RNA by recruitment of an RNA editing entity. Also provided herein are compositions, vectors, and cells comprising the engineered guide RNAs disclosed herein. Also provided herein are methods of introducing the engineered guide RNAs described herein into cells and methods of treating a disease or condition in a subject in need thereof comprising administering to the subject engineered guide RNAs described herein. |