http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022099270-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e06a12f3d96af2dab618b5ff07019f65 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
filingDate | 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d039a28ab5ab758fe1d5aafc1b452f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0142cc1e11fbe9985e3d63d2857de1b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_291fd65609eab1beeed7d430a9f2df15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64032c5df0bb5526c7aaf075d8a2e84e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_caf966463dcc50b385b29b6e0fcdea13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c634342af3ed588f522ad0c3595a606a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06e96b8a825a4c1df43ea75d50e8ec6c |
publicationDate | 2022-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2022099270-A1 |
titleOfInvention | Methods and systems for phasing sequencing strands and long‑range sequencing |
abstract | Described herein are methods synchronizing sequencing primers within a sequencing cluster and methods of generating long-range sequencing reads. The methods can include hybridizing primers to polynucleotide copies within a sequencing cluster; extending the primers through a first region of the polynucleotide copies using labeled nucleotides according to a sequencing flow order; extending the primers through a second region of the polynucleotide copies using one or more re-phasing flow steps that each include at least two different types of nucleotide bases; and extending the primers through a third region of the polynucleotide copies using labeled nucleotides according to the sequencing cycle. The rephasing flow steps may be initiated after a predetermined number of sequencing flow steps, after a measured sequencing signal strength falls below a predetermined sequencing signal strength threshold, or a measured sequencing signal-to-noise ratio falls below a sequencing signal-to-noise ratio threshold. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11763915-B2 |
priorityDate | 2020-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 162.