http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022071436-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a05a09d28a284fc55862088ef711de7e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K45-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K45-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate | 2021-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52e771982ef911b1225fb7a714e207fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7d5122cc1b83fc7ac562ca01cba5741 |
| publicationDate | 2022-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022071436-A1 |
| titleOfInvention | Method for measuring rna capable of detecting biological and physiological processes |
| abstract | Provided are: a method for detecting the aminoacylation profile of target RNA, particularly tRNA-functional RNA or small RNA, rapidly, with high accuracy, in a simple manner and at low cost; a method for screening for tRNA-functional RNA based on the aminoacylation profile thereof; a pharmaceutical composition for a disease; a gene therapy; a biomarker; and others. The present invention includes inventions each relied on a method for measuring a single nucleotide difference in a single molecule of target RNA qualitatively or quantitatively, in which the single nucleotide difference is the presence or absence of a nucleotide A in a 3'-terminal sequence, i.e., a CCA-CC pair, in the target RNA, and the method comprises (a-1) providing an RT primer comprising an adapter linked to the 3'-terminal of the target RNA and a sequence complementary to the sequence for the adapter, a specific amplification primer set, and a probe set that targets a single nucleotide difference, (b-1) linking the adapter to the target RNA by the action of an RNA ligase for the adapter to produce a linked product, and forming cDNA using the linked product as a template, and (β) amplifying a target region located on the cDNA and containing the single nucleotide difference by PCR and, at the same time, measuring the single nucleotide difference qualitatively or quantitatively. |
| priorityDate | 2020-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.