| abstract |
Provided is a method for catalytic synthesis of PAPS based on the construction of an ATP regeneration system, comprising artificially constructing a bifunctional PAPS synthesis enzyme by means of microbial recombinant expression. On the basis of the ATP regeneration system coupling the polyphosphate kinase derived from Corynebacterium glutamicum and Mycobacterium tuberculosis, two by-products, pyrophosphoric acid and ADP, can be recovered at the same time, the equivalent conversion of substrate and product can be achieved, the PAPS generated in the catalytic system has high purity, and the supply of sulfonic acid groups in most of sulfonic acid transfer reactions can be achieved. |