| abstract |
The present invention provides a kit and method for isothermal rapid detection of a SARS-CoV-2 virus nucleic acid. The detection method comprises: (a) providing a sample to be detected containing a target nucleic acid molecule; (b) mixing said sample with a cleavage reagent or a cleavage buffer solution containing the cleavage reagent so as to form a detection system, wherein the cleavage reagent comprises two guide ssDNAs, a gene editing enzyme (Ago), and a first reporter nucleic acid molecule, the first reporter nucleic acid molecule has a fluorescent group and a quenching group, and the two guide ssDNAs are adjacent to each other; and (c) performing fluorescence detection on the detection system so as to obtain a fluorescence signal value, and if the fluorescence signal value is detected in the detection system, indicating that the target nucleic acid molecule exists in said sample, and if the fluorescence signal value is not detected in the detection system, indicating that the target nucleic acid molecule does not exist in said sample. |