| abstract |
Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital polymerase chain reaction (dPCR). Systems and methods include those in which (1) two dyes have the same excitation wavelength range, but different emission wavelength ranges and/or (2) two dyes have the same emission wavelength range, but different excitation wavelength ranges. |