http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021250140-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d13c4f8c90522716555b78be5b79dbb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 |
| filingDate | 2021-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1394699c101d7d5580f11598b0f97205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30241a3c48d194e0869c6483362358cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c00df20d8b309a3be5fe1e291d8a614c |
| publicationDate | 2021-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2021250140-A1 |
| titleOfInvention | Isothermal real-time pcr method for determining presence of a pre-determined rna sequence in a sample |
| abstract | The present invention relates to a method for determining presence of a pre-determined RNA sequence in a sample, the method comprising the steps of adding one or more enzyme(s) providing activities of RNA- and DNA-dependent DNA polymerase activity and strand- displacement activity to the sample to be analysed for the presence of the pre-determined RNA sequence; simultaneously or subsequently adding at least five DNA primers to the sample to be analysed for the presence of the pre-determined RNA sequence, wherein at least one DNA primer comprises a sequence hybridisable to the RNA sequence and at least one DNA primer comprises a sequence hybridisable to the DNA sequence reverse-complementary to the RNA sequence; incubating the sample resulting from the previous steps at a fixed temperature; and determining whether an elongated double-stranded DNA sequence is present in the sample, wherein presence of the elongated double-stranded DNA sequence in the sample is indicative of the presence of the pre-determined RNA sequence in the sample wherein the pre-determined RNA sequence is part of an RNA virus and wherein no F3 primer is used. |
| priorityDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.