abstract |
Disclosed are a swab sample nucleic acid releaser and an application thereof. The sample releaser comprises 1-20 mol/L of metal ion chelating agent, 5-80 mmol/L of buffer solution with a pH value of 6-9, 5%-40% (v/v) of polar organic solvent, 0.2%-10% (v/v) of surfactant and 0.1-1 mol/L of nuclease inhibitor. The sample releaser may integrate three steps of sampling preservation, deactivation and nucleic acid extraction into one step, and the whole operation process from sampling to PCR detection is free from heating and midway lid opening, and operation is therefore simple. |