abstract |
Provided are a recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an amino acid dehydrogenase gene and/or activating the activity of transhydrogenase and/or NAD kinase, cell NADPH reducing power is increased, the yield and conversion rate of L-valine produced by Escherichia coli are improved, and one-step anaerobic fermentation of L-valine is achieved. |