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filingDate 2021-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2021195322-A1
titleOfInvention Methods for detecting low levels of covid-19 virus
abstract Provided herein is a method for detecting the presence of a COVID-19 virus in a human sample or an environmental sample having one or more viruses and bacterial pathogens. Samples are processed to obtain total nucleic acids. A combined reverse transcription and asymmetric PCR amplification reaction is performed to obtain fluorescent labeled COVID-19 virus specific amplicons. The amplicons are detected by microarray hybridization near the lowest limit of detection. Also provided is a method for detecting concurrently with COVID-19 virus, the presence of respiratory disease-causing pathogens including viruses, bacteria and fungus in a single assay using the above method.
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