abstract |
The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins ( e.g ., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided. |