| abstract |
The present disclosure is directed to an in vitro method for encapsulating animal tissue in an alginate-based hydrogel, termed ALTEN (Alginate-based Tissue Encapsulation). Encapsulated organoids produced using the ALTEN method retain cell viability e.g., during tissue culture and storage, and maintain tissue architecture and cell heterogeneity in the original tissue sample. The disclosure also relates to the use of ALTEN encapsulated tissues e.g., encapsulated tumour organoids, for drug-screening, in particular, in the context of personalised cancer therapies. |