| abstract |
The present invention relates to a method for detecting a pathogenic strain having resistance to carbapenem antibiotics in a biological sample. In the present invention, by directly confirming, through mass spectroscopy, carbapenem antibiotic degradation enzymes, particularly KPC, OXA, NDM, IMP, VIM, and/or GES protein(s), not only whether the pathogenic strain is resistant to antibiotics but also the type of resistance-related protein can be rapidly determined. In the present invention, by discovering in-vivo physical and chemical properties of respective enzymes, such as the cleavage length of a unique N-terminus according to the type of carbapenemase, and the oxidation and disulfide bond formation of methionine residues, and reflecting same in a reference mass value, the presence of an antibiotic-resistant strain can be more thoroughly detected with high reliability. Thus, the method can be effectively used in establishing strategies for appropriate antibiotic administration in the early stages of infection. |