http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021129016-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eac2f8eff077c4682236f6b66083d39a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-388 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 |
| filingDate | 2020-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f7f6eaf1e01e5baa33ed0b5a9debc2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3517d485104b0a958d909fc5c9be8be7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62d484c4c16b4155a2ed191441c94a13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcf164635da07a9082541f79507971ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07b0f80a872040cbadb32eb455601b70 |
| publicationDate | 2021-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2021129016-A1 |
| titleOfInvention | Method for desalting polypeptides |
| abstract | Disclosed is a method for desalting polypeptides, the method comprising loading a polypeptide sample onto a preparative column, rinsing the column with mobile phases A1 and B, then rinsing the column with mobile phases A2 and B, and finally performing gradient elution with A2 and B; collecting the sample; performing rotary evaporation under reduced pressure; and freeze-drying the product to obtain desalted refined polypeptides. In the present method, salt transfer can be performed on a reversed-phase high-efficiency preparative chromatography system by taking a silica filler with a bonded alkyl group as the stationary phase. The method solves the problem of it being difficult to elute samples during desalting without needing additional equipment and materials, and during salt transfer, can further remove impurities, improve sample purity, reduce sample volume after salt transfer, and increase sample concentration. The whole process is simple to operate and has a short production cycle, which is conducive to large-scale industrial production. |
| priorityDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.