| abstract |
Provided is an immobilized enzyme, a preparation method therefor, and an application thereof. The immobilized enzyme comprises an enzyme and an amino resin carrier for immobilizing the enzyme. The enzyme is selected from one of the following enzymes: transaminase, ketoreductase, monooxygenase, ammonia-lyase, alkene reductase, imine reductase, dehydrogenase, and nitrilase. The amino resin carrier is modified with a cross-linking agent treated with a polymer. By means of modifying the amino resin carrier with the cross-linking agent treated with the polymer, the enzyme immobilized on the amino resin carrier can easily form a cross-linking network, such that the immobilization effect of the enzyme is stable, thereby increasing the effectiveness of recycling the enzyme. |