abstract |
Provided are a highly active transposase and an application thereof, the amino acid sequence of the transposase is as shown in SEQ ID NO: 2 or 12, the transposase used for a transposon system can significantly improve the gene transfer activity of the transposon. The transposase enzyme and its coding nucleotide sequence can be used to construct a gene transfer system, to prepare or be used as a drug, preparation, or tool for genome research, gene therapy, cell therapy, or multi-functional stem cell induction and/or differentiation. |