| abstract |
Provided is a codon-optimized FAD-glucose dehydrogenase gene, derived from Aspergillus niger An76, the sequence thereof being as shown in SEQ ID No. 2. An initial FAD-glucose dehydrogenase gene cannot be successfully expressed in Pichia pastoris, but upon sequence optimization, can be successfully expressed in Pichia pastoris. In addition, a purified target protein can be obtained by means of separation and purification, and the recombinant FAD-glucose dehydrogenase may be used in glucose biosensors. |