http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021103812-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97a714fcb755e3262636236d7856f6b3 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12G1-0203 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12G1-022 |
filingDate | 2020-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_944dac8c7ae25e6055881b6123205c50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6233f67cb5164857c7b33d37969df9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c24ae5c4ea014e218843d32afd4a266 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0b4f25a1cc8bdec4f7aafcffae90a30 |
publicationDate | 2021-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2021103812-A1 |
titleOfInvention | Method for constructing oenococcus oeni engineering bacteria and use thereof |
abstract | Provided are a method for constructing Oenococcus oeni engineering bacteria and the use thereof. The construction method comprises the following steps: (1) preparing a lipase gene lipase; (2) preparing a homologous arm gene having the lipase gene; (3) preparing a plasmid pMG36e-lipase; (4) preparing a nisl fragment; (5) preparing a linear fragment containing no erythromycin sequence; (6) preparing an expression vector pMG36n-lipase; and (7) transforming the expression vector pMG36n-lipase into Oenococcus oeni competent cells to obtain the Oenococcus oeni engineering bacteria. The Oenococcus oeni engineering bacteria with the function of degrading esters obtained by means of the construction method can be applied to the production of wine, such that adverse effects on the taste and aroma of the wine caused by excessive esters in the wine-making process are avoided in order to reduce the preservation time of the wine and shorten the production cycle of the wine while achieving the same taste. |
priorityDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.