| abstract |
[Problem] To discover a method having greater stability, reproducibility, economicalness, and ease of operation by using human immortalized myeloid cells in an in vitro method for evaluating the safety of a substance. [Solution] A method for evaluating the skin sensitization capacity and/or pyrogenicity of a test substance, a method for detecting a skin sensitizing substance and/or pyrogenic substance in a sample, and a method for evaluating an effect on a function of immune cells in a sample using human immortalized myeloid cells, wherein the method includes measuring the amount of IL-6 and/or IL-8 produced in the culture broth of human immortalized myeloid cells. |