| abstract |
The present invention pertains to a method for making biological tissue transparent, the method being characterized by including: (i) a step for impregnating a biological tissue with water-soluble ethylenically unsaturated monomers before, during, or after fixing the biological tissue via a fixing agent, the water-soluble ethylenically unsaturated monomers including at least one type of water-soluble ethylenically unsaturated monomer that is zwitterionic, and forming a hydrogel by polymerizing the water-soluble ethylenically unsaturated monomers in the biological tissue; (ii) a step for fixing the biological tissue via the fixing agent before, during, or after the hydrogel forming step; and (iii) a step for removing lipids from the biological tissue after the fixing step. |