| abstract |
Provided are a chimeric antigen receptor cell library carrying gene element combination, preparation and screening method, and use thereof, the chimeric antigen receptor cell library is formed by fusion of cells and a carrier assembly, the carrier assembly carries three gene elements, and the three gene elements are respectively as follows: a plurality of first gene elements encoding one or more idiotypic chimeric antigen receptors; a second gene element carrying one or more gene circuits; and a third gene element encoding one or more inducible proteins. Wherein, the gene circuit is preprogrammed and is a combination of regulatory cis-acting elements and a transcription factors; and the inducible protein comprises one or two of drug resistance protein and suicide protein. By designing the chimeric antigen receptor library-genetic circuit-inducible protein coupling solution, the cell library and screening of complex and unknown disease target antigens are realized, so that the problems that antigens in abnormal antigen expression diseases are complex, diverse and easy to change, target identification is difficult and the like are solved, which has a wide application prospect. |