abstract |
Methods for inactivating in a cell a mutant allele of the elastase, neutrophil expressed gene (ELANE gene) gene having a mutation associated with severe congenital neutropenia (SCN) or cyclic neutropenia (CyN) and which cell is heterozygous at one or more polymorphic sites selected from the group consisting of: rs 10424470, rs4807932, rsl0414837, rs376107533, rs3761010, rs351108, rs3826946, rs 10413889, rs3761007, rs 10409474, rs3761005, rs351107, rs3761001, rs740021, rs781452480, rs371057361, rs570466264, rs 1041904080, rs7250194, rs 17216649, rs 199720952, rs6510983, rs 17223066, rs7255385, rs9749274, rslll361200, rsl 12639467, rsl41213775, rs28591229, rs 10469327, rs3834645, rsl683564, rs71335276, and rs8107095, the method comprising introducing to the cell a composition comprising a CRISPR nuclease or a sequence encoding the CRISPR nuclease; and a first RNA molecule comprising a guide sequence portion having 21-30 nucleotides, wherein a complex of the CRISPR nuclease and the first RNA molecule affects a double strand break in the mutant allele of the ELANE gene. |