| abstract |
According to the present invention, a phase difference image of an aggregate of pluripotent stem cells is generated from a hologram obtained by imaging the aggregate, a phase difference amount density is derived by dividing, by the volume of the aggregate, the total phase difference amount, which is a value obtained by adding the respective phase difference amounts of a plurality of pixels forming the phase difference image, and then pluripotent stem cells are sorted on the basis of the phase difference amount density. |