| abstract |
Provided is a recombinant adenovirus, which comprises a target expression cassette, a first homologous arm arranged at the 5' end of the target expression cassette, a second homologous arm arranged at the 3' end of the target expression cassette, and one or two sgRNA target sequences. The sgRNA target sequence is arranged at the 5' end of the first homologous arm or the 3' end of the second homologous arm or is respectively arranged at the 5' end of the first homologous arm and the 3' end of the second homologous arm. Further provided are a gene editing method based on the recombinant adenovirus, a method for preparing a gene-edited animal, a method for preparing engineered T cells, and a system, composition and kit comprising the recombinant adenovirus. |