Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c4322cb4de342be2dbedcbe0c278f6f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-16 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
filingDate |
2020-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56bc04a84f0489e9976c89e18f6b46ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea37f51182e8672b704033d3f30aca4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57f9dc62ca93ba7a2587440943ebb958 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90db4e5f992336e6c2086a3f174e780a |
publicationDate |
2021-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2021081325-A1 |
titleOfInvention |
Compositions, systems, and methods for high level expression of recombinant protein |
abstract |
Improved production of recombinant proteins in E. coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion in stationary phase. The process also provides an optimized autoinduction media, enabling routine batch production at various culture volumes where cells densities routinely reach ~ 5-7 g cell dry weight per liter and offer protein titers above 2 g/L. The methodology has been validated with a set of diverse heterologous proteins and is of general use for the facile optimization of routine protein expression from high throughput screens to fed-batch fermentation. |
priorityDate |
2019-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |