| abstract |
Disclosed is a high-efficiency synthesis and high-purity hyaluronic acid, and a recombinant corynebacterium glutamicum for an oligosaccharide thereof, relating to the technical field of bio-engineering. The yield of hyaluronic acid produced by the constructed recombinant corynebacterium glutamicum reaches 40 g/L, and the purity of the crude product reaches 95%. By means of a hyaluronic acid hydrolase from an external source and optimizing fermentation conditions, hyaluronic acid oligosaccharide of a specific molecular weight can be obtained, and further increasing of hyaluronic acid yield can be achieved, causing the yield to reach 72 g/L. |