| abstract |
The disclosure provides genetically-edited immune cells, methods of generating genetically-edited immune cells, and methods of therapy. In some embodiments, the methods described herein comprise contacting a plurality of mammalian cells with a polynucleic acid construct that comprises an insert sequence flanked by homology arms, wherein said homology arms comprise a sequence homologous to at most 400 consecutive nucleotides of a sequence adjacent to a target site in the genome of said plurality of mammalian cells. |