| abstract |
A phenol recognition SERS probe and preparation thereof, use thereof, and a SERS-based universal ultrasensitive immunoassay method. First, a phenol-responsive SERS probe is prepared by reducing chloroauric acid using DTDBA, and then ELISA is used in combination with SERS, a biomolecule is labelled with ALP, a substrate PPNa thereof is hydrolysed using ALP to produce phenol, and a biomolecule is detected sensitively by means of a SERS probe signal caused by phenol. The present invention can overcome the defect of low sensitivity of conventional enzyme-linked immunoassay; can solve the problem of enhancement and poor reproducibility of an SERS detection signal; has significant universality; can be widely used in immunoassay using ALP as enzyme marker to detect a wide variety of biomolecules; provides a solid foundation for the development of immunological technology on the bass of SERS detection; and has great development space and broad application prospects in the fields of biology, chemical detection, and medical diagnosis. |